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| Keywords: microbiology, bacteria, tooth, teeth, dental plaque, Streptococcus, denture, caries Type: diploma project Year: 2003 Discipline: Biochemistry, Biotechnology & Genetic Engineering |
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The project aimed to develop, test and assess a method for determining the plaque formation on three dental materials (amalgam, polyacrylate, ceramics) under aerobic conditions. This plaque formation is caused by microorganisms, which are present in the oral flora under normal conditions and which promote caries. Developing an adequate method for quantifying plaque formation is of special interest, mainly for improving common dental materials. In this way the risk of infection by dentures will be lowered. After a thorough screening of the composition of an artificial saliva solution and the selection of suitable specimen, an incubation of the three dental materials was performed under defined conditions. A significant growth of microorganisms, such as Streptococcus spp., could be detected in the artificial saliva solution. By means of ATP measurements the formation of biofilms of different concentrations could be detected after 4-6 weeks on each dental material. Additionally, it could be shown that the plaque developed most rapidly on polymer teeth and amalgam. However, it could also be proved that the plaque grew most prolifically on used denture teeth. The results of the project also demonstrate clearly, that the ATP measurements for quantifying vital microorganisms turned out to be a most efficient method. further information |
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